Analysis Note

ControlCONTROL ANTIGEN: Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Application

All procedures that are going to receive a full-length protein should be performed at 4°C, and the following protease inhibitor mixture should be used: pepstatin A (1 µg/mL), leupeptin (1 µg/mL), aprotinin (1 µg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 µg/mL each).

Western blot: 1:200 using ECL on rat brain membranes. Note: The addition of 2% Triton to the antibody is recommended for this lot number.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%)

Optimal working dilutions must be determined by the end user.

Anti-Sodium Channel Antibody, Voltage Gated, Brain Type II detects level of Sodium Channel & has been published & validated for use in IH & WB.

Research Sub CategoryIon Channels & Transporters

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified peptide of alpha subunit (amino acids 467-485) of rat type II voltage-gated sodium channel (VGSC) (Accession P04775).

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity Purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

ImmunoAffinity Purified

Specificity

Recognizes type II alpha subunit of VGSC. Does not cross react with any other sodium channel antigens tested so far.

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Target description

228 kDa